Journal: Journal of Orthopaedic Translation

Article Title: Pharmacological network analysis of the functions and mechanism of kaempferol from Du Zhong in intervertebral disc degeneration (IDD)

doi: 10.1016/j.jot.2023.01.002

Figure Lengend Snippet: Effects of Kaempferol IL-1β-induced ECM deposition NPCs were exposed to IL-1β stimulation (10 ​ng/ml, 24 ​h), treated with 10 ​μM kaempferol, and examined for the mRNA and protein levels of aggrecan, collagen II, SOX9, FN1 (A–B), MMP3, MMP13, ADAMTS-4, and ADAMTS-5 (C–D) using qRT-PCR and Immunoblotting, respectively. . One-way ANOVA followed by Tukey's post hoc test. N ​= ​3, ∗∗p ​< ​0.01, compared to PBS group; #p ​< ​0.05, ##p ​< ​0.01, compared with IL-1β group.

Article Snippet: The following primary antibodies were used in this study: p16 (CSB-PA003618, CUSABIO, Wuhan, China), p21 (ab109520, Abcam, Cambridge, UK), Rb (ab224426, Abcam), hTERT (DF7129, Affinity Biotech), Nrf2 (CSB-RA225569A0HU,Cusabio), HO-1 (10701-1-AP, Proteintech), NQO-1 (11451-1-AP, Proteintech), SOD1 (10269-1-AP, Proteintech), SOD2 (ab227091, Abcam), Cleaved-caspase 3 (ab2302, Abcam), Caspase 3 (ab13847, Abcam), Bax (50599-2-Ig, Proteintech), Bcl-2 (12789-1-AP, Proteintech), aggrecan (13880-1-AP, Proteintech), collagen II (CSB-PA005739ESR2HU, Cusabio), SOX9 (ab185966, Abcam), FN1 (AF5335, Affinity Biotech), MMP2 (CSB-PA003258, Cusabio), MMP3 (17873-1-AP, Proteintech), MMP13 (CSB-PA07029A0Rb, Cusabio), ADAMTS-4 (DF6986, Affinity Biotech), ADAMTS-5 (DF13268, Affinity Biotech), p-p38 (AF4001, Affinity Biotech), p38 (14064-1-AP, Proteintech), p-JNK (AF3318, Affinity Biotech), JNK (AF6319, Affinity Biotech), p-ERK1/2 (sc-81492, Santa Cruz, Dallas, DX, USA), ERK1/2 (16443-1-AP, Proteintech).

Techniques: Quantitative RT-PCR, Western Blot

Journal: Scientific Reports

Article Title: Perillyl alcohol promotes autophagy by suppressing the PI3K-AKT-mTOR signalling pathway in osteoarthritic chondrocytes in vitro

doi: 10.1038/s41598-025-12274-2

Figure Lengend Snippet: ( a ) mRNA expression of Col2a1 , Il-1β , and Mmp13 detected by qRT‒PCR. ( b ) Immunofluorescence staining and ( c ) quantitative analysis of COL2A1 and MMP13. Original magnification × 200, scale bar = 200 μm. ( d ) The expression of the proteins Aggrecan, COL2A1 and MMP13 was determined by Western blot and ( e ) quantitative analyses. Data from three independent experiments were evaluated, and the means ± SDs are shown. One-way ANOVA was used for statistical analyses, and multiple comparisons were performed for each group. NS indicates no significant difference, **/## indicates p <0.01, ***/### indicates p < 0.001.

Article Snippet: MMP13 , BOSTER , A00420.

Techniques: Expressing, Immunofluorescence, Staining, Western Blot

Journal: Frontiers in Pharmacology

Article Title: Modified Si Miao Powder granules alleviates osteoarthritis progression by regulating M1/M2 polarization of macrophage through NF-κB signaling pathway

doi: 10.3389/fphar.2024.1361561

Figure Lengend Snippet: MSMP affected cartilage matrix metabolism in OA mice. (A) Immunohistochemical staining of Col2a1. (B) Statistical analysis of the positive expression for Col2a1. (C) Immunohistochemical staining of Mmp13. (D) Statistical analysis of the positive expression for Mmp13. Scale bar represents 100 μm (Date are mean ± SD, #### p < 0.0001, versus Sham group; * p < 0.05, ** p < 0.01, **** p < 0.0001, versus OA group).

Article Snippet: The reagents in the current study were as follows: Lipopolysaccharide (Sigma), p65 Antibody (Abways, CY5034), p-p65 Antibody (Abways, CY6372), β-actin Antibody (Boster, BM3873), Col2a1 Antibody (Boster, BA0533), Mmp13 Antibody (Boster, BA2204), iNOS Antibody (Boster, BA0362), Arg1 Antibody (Servicebio, GB11285-100), IL-6 ELISA Kit (Boster), TNF-α ELISA Kit (Boster), IL-10 ELISA Kit (RuiXin Bio), HE Staining Kit (Solarbio), Senna O Solid Green Staining Kit (Solarbio) Animal Surgical Instruments, Absorbable Surgical Suture (6-0), Gavage Needle (12 gauge), Tissue Decalcification Solution (Bosterbio), Tissue Fixation Solution (Bosterbio), and Sodium Pentobarbital.

Techniques: Immunohistochemical staining, Staining, Expressing